Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PTPRE / PTP Epsilon Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107673-100
Catalog Number: LS-G107673
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PTP epsilon(PTPRE) (NM_130435), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HPTPE | PTPE | Ptpepsilon | R-PTP-EPSILON | PTPvarepsilon | PTPRE | PTP epsilon
- Origin Species
- Human
- NCBI GenBank
- NM_006504
- SwissProt
- P23469
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 74.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSNRSSFSRL TWFRKQRKAV VSTSDKKMPN GILEEQEQQR VMLLSRSPSG PKKYFPIPVE61HLEEEIRIRS ADDCKQFREE FNSLPSGHIQ GTFELANKEE NREKNRYPNI LPNDHSRVIL121SQLDGIPCSD YINASYIDGY KEKNKFIAAQ GPKQETVNDF WRMVWEQKSA TIVMLTNLKE181RKEEKCHQYW PDQGCWTYGN IRVCVEDCVV LVDYTIRKFC IQPQLPDGCK APRLVSQLHF241TSWPDFGVPF TPIGMLKFLK KVKTLNPVHA GPIVVHCSAG VGRTGTFIVI DAMMAMMHAE301QKVDVFEFVS RIRNQRPQMV QTDMQYTFIY QALLEYYLYG DTELDVSSLE KHLQTMHGTT361THFDKIGLEE EFRKLTNVRI MKENMRTGNL PANMKKARVI QIIPYDFNRV ILSMKRGQEY421TDYINASFID GYRQKDYFIA TQGPLAHTVE DFWRMIWEWK SHTIVMLTEV QEREQDKCYQ481YWPTEGSVTH GEITIEIKND TLSEAISIRD FLVTLNQPQA RQEEQVRVVR QFHFHGWPEI541GIPAEGKGMI DLIAAVQKQQ QQTGNHPITV HCSAGAGRTG TFIALSNILE RVKAEGLLDV601FQAVKSLRLQ RPHMVQTLEQ YEFCYKVVQD FIDIFSDYAN FKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
642 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.