Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PTPN7 / HEPTP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107843-100
Catalog Number: LS-G107843
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PTPN7(PTPN7) (NM_080588), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BPTP-4 | Dual specificity phosphatase 1 | HEPTP | LC-PTP | PTPNI | LPTP | PTPN7
- Origin Species
- Human
- NCBI GenBank
- NM_002832
- SwissProt
- P35236
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGASFWPIRQ AREQQRRALS FRQTSWLSEP PLGPAPHLSM VQAHGGRSRA QPLTLSLGAA61MTQPPPEKTP AKKHVRLQER RGSNVALMLD VRSLGAVEPI CSVNTPREVT LHFLRTAGHP121LTRWALQRQP PSPKQLEEEF LKIPSNFVSP EDLDIPGHAS KDRYKTILPN PQSRVCLGRA181QSQEDGDYIN ANYIRGYDGK EKVYIATQGP MPNTVSDFWE MVWQEEVSLI VMLTQLREGK241EKCVHYWPTE EETYGPFQIR IQDMKECPEY TVRQLTIQYQ EERRSVKHIL FSAWPDHQTP301ESAGPLLRLV AEVEESPETA AHPGPIVVHC SAGIGRTGCF IATRIGCQQL KARGEVDILG361IVCQLRLDRG GMIQTAEQYQ FLHHTLALYA GQLPEEPSPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
399 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.