Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PTPN6 / SHP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107839-100
Catalog Number: LS-G107839
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SHP1(PTPN6) (NM_080548), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HCP | HPTP1C | Hematopoietic cell phosphatase | PTP-1C | PTPN6 | SH-PTP1 | SHP-1L | SHP-1 | Shptp1 | HCPH | PTP1C | SHP1
- Origin Species
- Human
- NCBI GenBank
- NM_002831
- SwissProt
- P29350
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 67.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLSRGWFHRD LSGLDAETLL KGRGVHGSFL ARPSRKNQGD FSLSVRVGDQ VTHIRIQNSG61DFYDLYGGEK FATLTELVEY YTQQQGVLQD RDGTIIHLKY PLNCSDPTSE RWYHGHMSGG121QAETLLQAKG EPWTFLVRES LSQPGDFVLS VLSDQPKAGP GSPLRVTHIK VMCEGGRYTV181GGLETFDSLT DLVEHFKKTG IEEASGAFVY LRQPYYATRV NAADIENRVL ELNKKQESED241TAKAGFWEEF ESLQKQEVKN LHQRLEGQRP ENKGKNRYKN ILPFDHSRVI LQGRDSNIPG301SDYINANYIK NQLLGPDENA KTYIASQGCL EATVNDFWQM AWQENSRVIV MTTREVEKGR361NKCVPYWPEV GMQRAYGPYS VTNCGEHDTT EYKLRTLQVS PLDNGDLIRE IWHYQYLSWP421DHGVPSEPGG VLSFLDQINQ RQESLPHAGP IIVHCSAGIG RTGTIIVIDM LMENISTKGL481DCDIDIQKTI QMVRAQRSGM VQTEAQYKFI YVAIAQFIET TKKKLEVLQS QKGQESEYGN541ITYPPAMKNA HAKASRTSSK HKEDVYENLH TKNKREEKVK KQRSADKEKS KGSLKRKTRT601RPLEQKLISE EDLAANDILD YKDDDDKV
628 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.