Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PTPN5 / STEP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120426-100
Catalog Number: LS-G120426
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PTPN5(PTPN5) (NM_001039970), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PTPN5 | Striatal enriched phosphatase | Striatum enriched phosphatase | PTPSTEP | STEP
- Origin Species
- Human
- NCBI GenBank
- NM_006906
- SwissProt
- P54829
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNYEGARSER ENHAADDSEG GALDMCCSER LPGLPQPIVM EALDEAEGLQ DSQREMPPPP61PPSPPSDPAQ KPPPRGAGSH SLTVRSSLCL FAASQFLLEP TAWLDSGTWG VPSLLLVFLS121VGLVLVTTLV WHLLRTPPEP PTPLPPEDRR QSVSRQPSFT YSEWMEEKIE DDFLDLDPVP181ETPVFDCVMD IKPEADPTSL TVKSMGLQER RGSNVSLTLD MCTPGCNEEG FGYLMSPREE241SAREYLLSAS RVLQAEELHE KALDPFLLQA EFFEIPMNFV DPKEYDIPGL VRKNRYKTIL301PNPHSRVCLT SPDPDDPLSS YINANYIRGY GGEEKVYIAT QGPIVSTVAD FWRMVWQEHT361PIIVMITNIE EMNEKCTEYW PEEQVAYDGV EITVQKVIHT EDYRLRLISL KSGTEERGLK421HYWFTSWPDQ KTPDRAPPLL HLVREVEEAA QQEGPHCAPI IVHCSAGIGR TGCFIATSIC481CQQLRQEGVV DILKTTCQLR QDRGGMIQTC EQYQFVHHVM SLYEKQLSHQ SPETRTRPLEQKLISEEDLAANDILDYKDDDDKV
533 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.