Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PTK2B / PYK2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105361-100
Catalog Number: LS-G105361
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PYK2(PTK2B) (NM_173175), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CADTK | CAKB | Cell adhesion kinase beta | FADK 2 | FADK2 | Focal adhesion kinase 2 | FRNK | FAK2 | Proline-rich tyrosine kinase 2 | Protein-tyrosine kinase 2-beta | Protein kinase B | Protein tyrosine kinase 2 beta | PTK2B | PYK2 | RAFTK | CAK-beta | PKB | PTK
- Origin Species
- Human
- NCBI GenBank
- NM_004103
- SwissProt
- Q14289
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 111 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSGVSEPLSR VKLGTLRRPE GPAEPMVVVP VDVEKEDVRI LKVCFYSNSF NPGKNFKLVK61CTVQTEIREI ITSILLSGRI GPNIRLAECY GLRLKHMKSD EIHWLHPQMT VGEVQDKYEC121LHVEAEWRYD LQIRYLPEDF MESLKEDRTT LLYFYQQLRN DYMQRYASKV SEGMALQLGC181LELRRFFKDM PHNALDKKSN FELLEKEVGL DLFFPKQMQE NLKPKQFRKM IQQTFQQYAS241LREEECVMKF FNTLAGFANI DQETYRCELI QGWNITVDLV IGPKGIRQLT SQDAKPTCLA301EFKQIRSIRC LPLEEGQAVL QLGIEGAPQA LSIKTSSLAE AENMADLIDG YCRLQGEHQG361SLIIHPRKDG EKRNSLPQIP MLNLEARRSH LSESCSIESD IYAEIPDETL RRPGGPQYGI421AREDVVLNRI LGEGFFGEVY EGVYTNHKGE KINVAVKTCK KDCTLDNKEK FMSEAVIMKN481LDHPHIVKLI GIIEEEPTWI IMELYPYGEL GHYLERNKNS LKVLTLVLYS LQICKAMAYL541ESINCVHRDI AVRNILVASP ECVKLGDFGL SRYIEDEDYY KASVTRLPIK WMSPESINFR601RFTTASDVWM FAVCMWEILS FGKQPFFWLE NKDVIGVLEK GDRLPKPDLC PPVLYTLMTR661CWDYDPSDRP RFTELVCSLS DVYQMEKDIA MEQERNARYR TPKILEPTAF QEPPPKPSRP721KYRPPPQTNL LAPKLQFQEE DFIQPSSREE AQQLWEAEKV KMRQILDKQQ KQMVEDYQWL781RQEEKSLDPM VYMNDKSPLT PEKEVGYLEF TGPPQKPPRL GAQSIQPTAN LDRTDDLVYL841NVMELVWAVL ELKNELCQLP PEGYVVVVKN VGLTLRKLIG SVDDLLPSLP SSSRTEIEGT901QKLLNKDLAE LINKMRLAQQ NAVTSLSEEC KRQMLTASHT LAVDAKNLLD AVDQAKVLAN961LAHPPAETRTRPLEQKLISEEDLAANDILDYKDDDDKV
967 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.