LSBio
PTGES3L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PTGES3L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PTGES3L antibodies →
$504.00each
Catalog Number: LS-G125630-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AARSD1(PTGES3L) (NM_001136042), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001142653

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFSLPLNCSP DHIRRGSCWG RPQDLKIAAP AWNSKCHPGA GAAMARQHAR TLWYDRPRYV
61FMEFCVEDST DVHVLIEDHR IVFSCKNADG VELYNEIEFY AKVNSKDSQD KRSSRSITCF
121VRKWKEKVAW PRLTKEDIKP VWLSVDFDNW RDWEGDEEME LAHVEHYAEL LKKVSTKRPP
181PAMDDLDFTT TVVSCCPAEL QTEGSNGKKE VLSGFQVVLE DTVLFPEGGG QPDDRGTIND
241ISVLRVTRRG EQADHFTQTP LDPGSQVLVR VDWERRFDHM QQHSGQHLIT AVADHLFKLK
301TTSWELGRFR SAIELDTPSM TAEQVAAIEQ SVNEKIRDRL PVNVRELSLD DPEVEQVSGR
361GLPDDHAGPI RVVNIEGVDS NMCCGTHVSN LSDLQVIKIL GTEKGKKNRT NLIFLSGNRV
421LKWMERSHGT EKALTALLKC GAEDHVEAVK KLQNSTKILQ KNNLNLLRDL AVHIAHSLRN
481SPDWGGVVIL HRKEGDSEFM NIIANEIGSE ETLLFLTVGD EKGGGLFLLA GPPASVETLG
541PRVAEVLEGK GAGKKGRFQG KATKMSRRME AQALLQDYIS TQSAKE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

586 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.