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PTDSS2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PTDSS2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PTDSS2 antibodies →
$504.00each
Catalog Number: LS-G101965-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PTDSS2 (NM_030783), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PSS-2 | Phosphatidylserine synthase 2 | PSS2 | PtdSer synthase 2 | PTDSS2 | Serine-exchange enzyme II
Origin Species
Human
NCBI GenBank
NM_030783
SwissProt
Q9BVG9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MRRGERRDAG GPRPESPVPA GRASLEEPPD GPSAGQATGP GEGRRSTESE VYDDGTNTFF
61WRAHTLTVLF ILTCTLGYVT LLEETPQDTA YNTKRGIVAS ILVFLCFGVT QAKDGPFSRP
121HPAYWRFWLC VSVVYELFLI FILFQTVQDG RQFLKYVDPK LGVPLPERDY GGNCLIYDPD
181NETDPFHNIW DKLDGFVPAH FLGWYLKTLM IRDWWMCMII SVMFEFLEYS LEHQLPNFSE
241CWWDHWIMDV LVCNGLGIYC GMKTLEWLSL KTYKWQGLWN IPTYKGKMKR IAFQFTPYSW
301VRFEWKPASS LRRWLAVCGI ILVFLLAELN TFYLKFVLWM PPEHYLVLLR LVFFVNVGGV
361AMREIYDFMD DPKPHKKLGP QAWLVAAITA TELLIVVKYD PHTLTLSLPF YISQCWTLGS
421VLALTWTVWR FFLRDITLRY KETRWQKWQN KDDQGSTVGN GDQHPLGLDE DLLGPGVAEG
481EGAPTPN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

487 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.