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PTCHD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PTCHD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G120521-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PTCHD3 (NM_001034842), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Patched domain containing 3 | Patched-related protein | PTCHD3 | PTR
Origin Species
Human
SwissProt
Q3KNS1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPWVEPKPRP GPEQKPKLTK PDSATGPQWY QESQESESEG KQPPPGPLAP PKSPEPSGPL
61ASEQDAPLPE GDDAPPRPSM LDDAPRLPLE LDDAPLPEEE TPEPTAICRH RHRCHTDCLE
121GLLSRTFQWL GWQVGAHPWI FLLAPLMLTA APGTGFLYLP KDEEEDLEEH YTPVGSPAKA
181ERRFVQGHFT TNDSYRFSAS RRSTEANFVS LLVVSYSDSL LDPATFAEVS KLDGAVQDLR
241VAREKGSQIQ YQQVCARYRA LCVPPNPILY AWQVNKTLNL SSISFPAYNH GRHPLYLTGF
301FGGYILGGSL GMGQLLLRAK AMRLLYYLKT EDPEYDVQSK QWLTHLLDQF TNIKNILALK
361KIEVVHFTSL SRQLEFEATS VTVIPVFHLA YILIILFAVT SCFRFDCIRN KMCVAAFGVI
421SAFLAVVSGF GLLLHIGVPF VIIVANSPFL ILGVGVDDMF IMISAWHKTN LADDIRERMS
481NVYSKAAVSI TITTITNILA LYTGIMSSFR SVQCFCIYTG MTLLFCYFYN ITCFGAFMAL
541DGKREVVCLC WLKKADPKWP SFKKFCCFPF GSVPDEHGTD IHPMSLFFRD YFGPFLTRSE
601SKYFVVFIYV LYIISSIYGC FHVQEGLDLR NLASDDSYIT PYFNVEENYF SDYGPRVMVI
661VTKKVDYWDK DVRQKLENCT KIFEKNVYVD KNLTEFWLDA YVQYLKGNSQ DPNEKNTFMN
721NIPDFLSNFP NFQHDINISS SNEIISSRGF IQTTDVSSSA KKKILLF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

767 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.