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PTCD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PTCD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PTCD3 antibodies →
$504.00each
Catalog Number: LS-G101517-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PTCD3 (NM_017952), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PTCD3 | TRG-15
Origin Species
Human
NCBI GenBank
NM_017952
SwissProt
Q96EY7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVVSAVRWL GLRSRLGQPL TGRRAGLCEQ ARSCRFYSGS ATLSKVEGTD VTGIEEVVIP
61KKKTWDKVAV LQALASTVNR DTTAVPYVFQ DDPYLMPASS LESRSFLLAK KSGENVAKFI
121INSYPKYFQK DIAEPHIPCL MPEYFEPQIK DISEAALKER IELRKVKASV DMFDQLLQAG
181TTVSLETTNS LLDLLCYYGD QEPSTDYHFQ QTGQSEALEE ENDETSRRKA GHQFGVTWRA
241KNNAERIFSL MPEKNEHSYC TMIRGMVKHR AYEQALNLYT ELLNNRLHAD VYTFNALIEA
301TVCAINEKFE EKWSKILELL RHMVAQKVKP NLQTFNTILK CLRRFHVFAR SPALQVLREM
361KAIGIEPSLA TYHHIIRLFD QPGDPLKRSS FIIYDIMNEL MGKRFSPKDP DDDKFFQSAM
421SICSSLRDLE LAYQVHGLLK TGDNWKFIGP DQHRNFYYSK FFDLICLMEQ IDVTLKWYED
481LIPSAYFPHS QTMIHLLQAL DVANRLEVIP KIWKDSKEYG HTFRSDLREE ILMLMARDKH
541PPELQVAFAD CAADIKSAYE SQPIRQTAQD WPATSLNCIA ILFLRAGRTQ EAWKMLGLFR
601KHNKIPRSEL LNELMDSAKV SNSPSQAIEV VELASAFSLP ICEGLTQRVM SDFAINQEQK
661EALSNLTALT SDSDTDSSSD SDSDTSEGK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

689 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.