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PTCD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PTCD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PTCD1 antibodies →
$504.00each
Catalog Number: LS-G101331-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PTCD1 (NM_015545), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0632 | PTCD1
Origin Species
Human
NCBI GenBank
NM_015545
SwissProt
O75127

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDFVRLARLF ARARPMGLFI LQHLDPCRAR WAGGREGLMR PMWAPFSSSS SQLPLGQERQ
61ENTGSLGSDP SHSNSTATQE EDEEEEESFG TLSDKYSSRR LFRKSAAQFH NLRFGERRDE
121QMEPEPKLWR GRRNTPYWYF LQCKHLIKEG KLVEALDLFE RQMLKEERLQ PMESNYTVLI
181GGCGRVGYLK KAFNLYNQMK KRDLEPSDAT YTALFNVCAE SPWKDSALQS ALKLRQQLQA
241KNFELNLKTY HALLKMAAKC ADLRMCLDVF KEIIHKGHVV TEETFSFLLM GCIQDKKTGF
301RYALQVWRLM LSLGLQPSRD SYNLLLVAAR DCGLGDPQVA SELLLKPREE ATVLQPPVSR
361QRPRRTAQAK AGNLMSAMLH VEALERQLFL EPSQALGPPE PPEARVPGKA QPEVDTKAEP
421SHTAALTAVA LKPPPVELEV NLLTPGAVPP TVVSFGTVTT PADRLALIGG LEGFLSKMAE
481HRQQPDIRTL TLLAEVVESG SPAESLLLAL LDEHQVEADL TFFNTLVRKK SKLGDLEGAK
541ALLPVLAKRG LVPNLQTFCN LAIGCHRPKD GLQLLTDMKK SQVTPNTHIY SALINAAIRK
601LNYTYLISIL KDMKQNRVPV NEVVIRQLEF AAQYPPTFDR YQGKNTYLEK IDGFRAYYKQ
661WLTVMPAEET PHPWQKFRTK PQGDQDTGKE ADDGCALGGR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

700 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.