Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PTBP3 / ROD1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G116139-100
Catalog Number: LS-G116139
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ROD1(PTBP3) (NM_005156), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ROD1 | PTBP3 | Regulator of differentiation 1
- Origin Species
- Human
- NCBI GenBank
- NM_005156
- SwissProt
- O95758
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDGVVTDLIT VGLKRGSDEL LSSGIINGPF TMNSSTPSTA NGNDSKKFKR DRPPCSPSRV61LHLRKIPCDV TEAEIISLGL PFGKVTNLLM LKGKSQAFLE MASEEAAVTM VNYYTPITPH121LRSQPVYIQY SNHRELKTDN LPNQARAQAA LQAVSAVQSG SLALSGGPSN EGTVLPGQSP181VLRIIIENLF YPVTLEVLHQ IFSKFGTVLK IITFTKNNQF QALLQYADPV NAHYAKMALD241GQNIYNACCT LRIDFSKLTS LNVKYNNDKS RDFTRLDLPT GDGQPSLEPP MAAAFGAPGI301ISSPYAGAAG FAPAIGFPQA TGLSVPAVPG ALGPLTITSS AVTGRMAIPG ASGIPGNSVL361LVTNLNPDLI TPHGLFILFG VYGDVHRVKI MFNKKENALV QMADANQAQL AMNHLSGQRL421YGKVLRATLS KHQAVQLPRE GQEDQGLTKD FSNSPLHRFK KPGSKNFQNI FPPSATLHLS481NIPPSVTVDD LKNLFIEAGC SVKAFKFFQK DRKMALIQLG SVEEAIQALI ELHNHDLGEN541HHLRVSFSKS TITRTRPLEQKLISEEDLAANDILDYKDDDDKV
552 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.