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PSMD5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PSMD5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PSMD5 antibodies →
$504.00each
Catalog Number: LS-G100448-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PSMD5 (NM_005047), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
26S protease subunit S5 basic | 26s proteasome subunit 5b | 26S proteasome subunit S5B | KIAA0072 | S5B | Pmsd5 | PSMD5
Origin Species
Human
NCBI GenBank
NM_005047
SwissProt
Q16401

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAQALALLR EVARLEAPLE ELRALHSVLQ AVPLNELRQQ AAELRLGPLF SLLNENHREK
61TTLCVSILER LLQAMEPVHV ARNLRVDLQR GLIHPDDSVK ILTLSQIGRI VENSDAVTEI
121LNNAELLKQI VYCIGGENLS VAKAAIKSLS RISLTQAGLE ALFESNLLDD LKSVMKTNDI
181VRYRVYELII EISSVSPESL NYCTTSGLVT QLLRELTGED VLVRATCIEM VTSLAYTHHG
241RQYLAQEGVI DQISNIIVGA DSDPFSSFYL PGFVKFFGNL AVMDSPQQIC ERYPIFVEKV
301FEMIESQDPT MIGVAVDTVG ILGSNVEGKQ VLQKTGTRFE RLLMRIGHQS KNAPVELKIR
361CLDAISSLLY LPPEQQTDDL LRMTESWFSS LSRDPLELFR GISSQPFPEL HCAALKVFTA
421IANQPWAQKL MFNSPGFVEY VVDRSVEHDK ASKDAKYELV KALANSKTIA EIFGNPNYLR
481LRTYLSEGPY YVKPVSTTAV EGAE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

504 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.