Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PSMD12 / Rpn5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G127502-100
Catalog Number: LS-G127502
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human 26S proteasome non ATPase regulatory subunit 12(PSMD12) (NM_174871), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PSMD12 | Rpn5 | p55
- Origin Species
- Human
- NCBI GenBank
- NM_002816
- SwissProt
- O00232
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MADGGSERAD GRIVKMEVDY SATVDQRLPE CAKLAKASDM VSTSRILVAV VKMCYEAKEW61DLLNENIMLL SKRRSQLKQA VAKMVQQCCT YVEEITDLPI KLRLIDTLRM VTEGKIYVEI121ERARLTKTLA TIKEQNGDVK EAASILQELQ VETYGSMEKK ERVEFILEQM RLCLAVKDYI181RTQIISKKIN TKFFQEENTE KLKLKYYNLM IQLDQHEGSY LSICKHYRAI YDTPCIQAES241EKWQQALKSV VLYVILAPFD NEQSDLVHRI SGDKKLEEIP KYKDLLKLFT TMELMRWSTL301VEDYGMELRK GSLESPATDV FGSTEEGEKR WKDLKNRVVE HNIRIMAKYY TRITMKRMAQ361LLDLSVDESE AFLSNLVVNK TIFAKVDRLA GIINFQRPKD PNNLLNDWSQ KLNSLMSLVN421KTTHLIAKEE MIHNLQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
436 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.