Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PSMB8 / LMP7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116834-100
Catalog Number: LS-G116834
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PSMB8 (NM_004159), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- D6S216 | ALDD | Low molecular mass protein 7 | LMP7 | JMP | Proteasome subunit beta 5i | Proteasome subunit beta type-8 | PSMB5i | Macropain subunit C13 | Y2 | NKJO | Protease component C13 | Proteasome component C13 | Proteasome subunit beta-5i | Proteasome-related gene 7 | RING10 | Beta5i | D6S216E | Low molecular weight protein 7 | Proteasome subunit Y2 | PSMB8
- Origin Species
- Human
- NCBI GenBank
- NM_004159
- SwissProt
- P28062
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 29.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLIGTPTPRD TTPSSWLTSS LLVEAAPLDD TTLPTPVSSG CPGLEPTEFF QSLGGDGERN61VQIEMAHGTT TLAFKFQHGV IAAVDSRASA GSYISALRVN KVIEINPYLL GTMSGCAADC121QYWERLLAKE CRLYYLRNGE RISVSAASKL LSNMMCQYRG MGLSMGSMIC GWDKKGPGLY181YVDEHGTRLS GNMFSTGSGN TYAYGVMDSG YRPNLSPEEA YDLGRRAIAY ATHRDSYSGG241VVNMYHMKED GWVKVESTDV SDLLHQYREA NQTRTRPLEQ KLISEEDLAA NDILDYKDDD301DKV
303 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.