Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PSMB11 / BETA5T Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G119160-100
Catalog Number: LS-G119160
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PSMB11 (NM_001099780), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BETA5T | Proteasome subunit beta 5T | Proteasome subunit beta-5t | PSMB11
- Origin Species
- Human
- SwissProt
- A5LHX3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 32.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MALQDVCKWQ SPDTQGPSPH LPRAGGWAVP RGCDPQTFLQ IHGPRLAHGT TTLAFRFRHG61VIAAADTRSS CGSYVACPAS CKVIPVHQHL LGTTSGTSAD CATWYRVLQR ELRLRELREG121QLPSVASAAK LLSAMMSQYR GLDLCVATAL CGWDRSGPEL FYVYSDGTRL QGDIFSVGSG181SPYAYGVLDR GYRYDMSTQE AYALARCAVA HATHRDAYSG GSVDLFHVRE SGWEHVSRSD241ACVLYVELQK LLEPEPEEDA SHAHPEPATA HRAAEDRELS VGPGEVTPGD SRMPAGTETVSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
300 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.