Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PSK / SEZ6L2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114453-100
Catalog Number: LS-G114453
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SEZ6L2 (NM_012410), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PSK | PSK-1 | SEZ6L2 | Seizure 6-like protein 2
- Origin Species
- Human
- NCBI GenBank
- NM_012410
- SwissProt
- Q6UXD5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 92.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGTPRAQHPP PPQLLFLILL SCPWIQGLPL KEEEILPEPG SETPTVASEA LAELLHGALL61RRGPEMGYLP GPPLGPEGGE EETTTTIITT TTVTTTVTSP VLCNNNISEG EGYVESPDLG121SPVSRTLGLL DCTYSIHVYP GYGIEIQVQT LNLSQEEELL VLAGGGSPGL APRLLANSSM181LGEGQVLRSP TNRLLLHFQS PRVPRGGGFR IHYQAYLLSC GFPPRPAHGD VSVTDLHPGG241TATFHCDSGY QLQGEETLIC LNGTRPSWNG ETPSCMASCG GNIHNATLGR IVSPEPGGAV301GPNLTCRWVI EAAEGRRLHL HFERVSLDED NDRLMVRSGG SPLSPVIYDS DMDDVPERGL361ISDAQSLYVE LLSETPANPL LLSLRFEAFE EDRCFAPFLA HGNVTTTDPE YRPGALATFS421CLPGYALEPP GPPNAIECVD PTEPHWNDTE PACKAMCGGE LSEPAGVVLS PDWPQSYSPG481QDCVWGVHVQ EEKRILLQVE ILNVREGDML TLFDGDGPSA RVLAQLRGPQ PRRRLLSSGP541DLTLQFQAPP GPPNPGLGQG FVLHFKEVPR NDTCPELPPP EWGWRTASHG DLIRGTVLTY601QCEPGYELLG SDILTCQWDL SWSAAPPACQ KIMTCADPGE IANGHRTASD AGFPVGSHVQ661YRCLPGYSLE GAAMLTCYSR DTGTPKWSDR VPKCALKYEP CLNPGVPENG YQTLYKHHYQ721AGESLRFFCY EGFELIGEVT ITCVPGHPSQ WTSQPPLCKV AYEELLDNRK LEVTQTTDPS781RQLEGGNLAL AILLPLGLVI VLGSGVYIYY TKLQGKSLFG FSGSHSYSPI TVESDFSNPL841YEAGDTREYE VSITRTRPLEQKLISEEDLAANDILDYKDDDDKV
853 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.