Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PSG1 / CD66f Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129779-100
Catalog Number: LS-G129779
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Pregnancy Specific Glycoprotein 1(PSG1) (NM_001184826), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- B1G1 | CD66f | CD66f antigen | FL-NCA-1/2 | PS-beta-G-1 | PSBG1 | PSG1 | PSG95 | PSGGA | PSGIIA | PSBG-1 | DHFRP2 | PS-beta-C/D
- Origin Species
- Human
- NCBI GenBank
- NM_006905
- SwissProt
- P11464
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGTLSAPPCT QRIKWKGLLL TASLLNFWNL PTTAQVTIEA EPTKVSEGKD VLLLVHNLPQ61NLTGYIWYKG QMRDLYHYIT SYVVDGEIII YGPAYSGRET AYSNASLLIQ NVTREDAGSY121TLHIIKGDDG TRGVTGRFTF TLHLETPKPS ISSSNLNPRE TMEAVSLTCD PETPDASYLW181WMNGQSLPMT HSLKLSETNR TLFLLGVTKY TAGPYECEIR NPVSASRSDP VTLNLLPKLP241KPYITINNLN PRENKDVLNF TCEPKSENYT YIWWLNGQSL PVSPRVKRPI ENRILILPSV301TRNETGPYQC EIRDRYGGIR SDPVTLNVLY GPDLPRIYPS FTYYRSGEVL YLSCSADSNP361PAQYSWTINE KFQLPGQKLF IRHITTKHSG LYVCSVRNSA TGKESSKSMT VEVSEALTRTRPLEQKLISEEDLAANDILDYKDDDDKV
417 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.