PSAP / Prosaposin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119528-100
Catalog Number: LS-G119528
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PSAP (NM_001042465), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cerebroside sulfate activator | GLBA | Proactivator polypeptide | PSAP | Prosaposin | SAP1
- Origin Species
- Human
- NCBI GenBank
- NM_002778
- SwissProt
- P07602
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.48 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MYALFLLASL LGAALAGPVL GLKECTRGSA VWCQNVKTAS DCGAVKHCLQ TVWNKPTVKS61LPCDICKDVV TAAGDMLKDN ATEEEILVYL EKTCDWLPKP NMSASCKEIV DSYLPVILDI121IKGEMSRPGE VCSALNLCES LQKHLAELNH QKQLESNKIP ELDMTEVVAP FMANIPLLLY181PQDGPRSKPQ PKDNGDVCQD CIQMVTDIQT AVRTNSTFVQ ALVEHVKEEC DRLGPGMADI241CKNYISQYSE IAIQMMMHMQ DQQPKEICAL VGFCDEVKEM PMQTLVPAKV ASKNVIPALE301LVEPIKKHEV PAKSDVYCEV CEFLVKEVTK LIDNNKTEKE ILDAFDKMCS KLPKSLSEEC361QEVVDTYGSS ILSILLEEVS PELVCSMLHL CSGTRLPALT VHVTQPKDGG FCEVCKKLVG421YLDRNLEKNS TKQEILAALE KGCSFLPDPY QKQCDQFVAE YEPVLIEILV EVMDPSFVCL481KIGACPSAHK PLLGTEKCIW GPSYWCQNTE TAAQCNAVEH CKRHVWNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
527 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.