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PRTG Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PRTG Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PRTG antibodies →
$725.00each
Catalog Number: LS-G105163-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRTG (NM_173814), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IGDCC5 | PRTG | Protein Shen-Dan | Protogenin | Shen-dan
Origin Species
Human
SwissProt
Q2VWP7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
126.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPPLRPLAR LRPPGMLLRA LLLLLLLSPL PGVWCFSELS FVKEPQDVTV TRKDPVVLDC
61QAHGEVPIKV TWLKNGAKMS ENKRIEVLSN GSLYISEVEG RRGEQSDEGF YQCLAMNKYG
121AILSQKAHLA LSTISAFEVQ PISTEVHEGG VARFACKISS HPPAVITWEF NRTTLPMTMD
181RITALPTGVL QIYDVSQRDS GNYRCIAATV AHRRKSMEAS LTVIPAKESK SFHTPTIIAG
241PQNITTSLHQ TVVLECMATG NPKPIISWSR LDHKSIDVFN TRVLGNGNLM ISDVRLQHAG
301VYVCRATTPG TRNFTVAMAT LTVLAPPSFV EWPESLTRPR AGTARFVCQA EGIPSPKMSW
361LKNGRKIHSN GRIKMYNSKL VINQIIPEDD AIYQCMAENS QGSILSRARL TVVMSEDRPS
421APYNVHAETM SSSAILLAWE RPLYNSDKVI AYSVHYMKAE GLNNEEYQVV IGNDTTHYII
481DDLEPASNYT FYIVAYMPMG ASQMSDHVTQ NTLEDVPLRP PEISLTSRSP TDILISWLPI
541PAKYRRGQVV LYRLSFRLST ENSIQVLELP GTTHEYLLEG LKPDSVYLVR ITAATRVGLG
601ESSVWTSHRT PKATSVKAPK SPELHLEPLN CTTISVRWQQ DVEDTAAIQG YKLYYKEEGQ
661QENGPIFLDT KDLLYTLSGL DPRRKYHVRL LAYNNIDDGY QADQTVSTPG CVSVRDRMVP
721PPPPPHHLYA KANTSSSIFL HWRRPAFTAA QIINYTIRCN PVGLQNASLV LYLQTSETHM
781LVQGLEPNTK YEFAVRLHVD QLSSPWSPVV YHSTLPEAPA GPPVGVKVTL IEDDTALVSW
841KPPDGPETVV TRYTILYASR KAWIAGEWQV LHREGAITMA LLENLVAGNV YIVKISASNE
901VGEGPFSNSV ELAVLPKETS ESNQRPKRLD SADAKVYSGY YHLDQKSMTG IAVGVGIALT
961CILICVLILI YRSKARKSSA SKTAQNGTQQ LPRTSASLAS GNEVGKNLEG AVGNEESLMP
1021MIMPNSFIDA KGGTDLIINS YGPIIKNNSK KKWFFFQDSK KIQVEQPQRR FTPAVCFYQP
1081GTTVLISDED SPSSPGQTTS FSRPFGVAAD TEHSANSEGS HETGDSGRFS HESNDEIHLS
1141SVISTTPPNL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1150 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.