LSBio
PRSS56 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PRSS56 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PRSS56 antibodies →
$504.00each
Catalog Number: LS-G131051-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRSS56 (NM_001195129), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001195129

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLLAVLLLLP LPSSWFAHGH PLYTRLPPSA LQVLSAQGTQ ALQAAQRSAQ WAINRVAMEI
61QHRSHECRGS GRPRPQALLQ DPPEPGPCGE RRPSTANVTR AHGRIVGGSA APPGAWPWLV
121RLQLGGQPLC GGVLVAASWV LTAAHCFVGA PNELLWTVTL AEGSRGEQAE EVPVNRILPH
181PKFDPRTFHN DLALVQLWTP VSPGGSARPV CLPQEPQEPP AGTACAIAGW GALFEDGPEA
241EAVREARVPL LSTDTCRRAL GPGLRPSTML CAGYLAGGVD SCQGDSGGPL TCSEPGPRPR
301EVLFGVTSWG DGCGEPGKPG VYTRVAVFKD WLQEQMSASS SREPSCRELL AWDPPQELQA
361DAARLCAFYA RLCPGSQGAC ARLAHQQCLQ RRRRCELRSL AHTLLGLLRN AQELLGPRPG
421LRRLAPALAL PAPALRESPL HPARELRLHS GSRAAGTRFP KRRPEPRGEA NGCPGLEPLR
481QKLAALQGAH AWILQVPSEH LAMNFHEVLA DLGSKTLTGL FRAWVRAGLG GRHVAFSGLV
541GLEPATLARS LPRLLVQALQ AFRVAALAEG EPEGPWMDVG QGPGLERKGH HPLNPQVPPA
601RQP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

603 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.