LSBio
PRSS35 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PRSS35 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PRSS35 antibodies →
$504.00each
Catalog Number: LS-G105804-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRSS35 (NM_153362), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C6orf158 | DJ223E3.1 | Inactive serine protease 35 | Protease | serine | 35 | PRSS35
Origin Species
Human
NCBI GenBank
NM_153362

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
46.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MENMLLWLIF FTPGWTLIDG SEMEWDFMWH LRKVPRIVSE RTFHLTSPAF EADAKMMVNT
61VCGIECQKEL PTPSLSELED YLSYETVFEN GTRTLTRVKV QDLVLEPTQN ITTKGVSVRR
121KRQVYGTDSR FSILDKRFLT NFPFSTAVKL STGCSGILIS PQHVLTAAHC VHDGKDYVKG
181SKKLRVGLLK MRNKSGGKKR RGSKRSRREA SGGDQREGTR EHLQERAKGG RRRKKSGRGQ
241RIAEGRPSFQ WTRVKNTHIP KGWARGGMGD ATLDYDYALL ELKRAHKKKY MELGISPTIK
301KMPGGMIHFS GFDNDRADQL VYRFCSVSDE SNDLLYQYCD AESGSTGSGV YLRLKDPDKK
361NWKRKIIAVY SGHQWVDVHG VQKDYNVAVR ITPLKYAQIC LWIHGNDANC AYG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

413 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.