Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PRSS3 / Trypsin 3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G127029-100
Catalog Number: LS-G127029
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Trypsin(PRSS3) (NM_007343), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Brain trypsinogen | Mesotrypsin | PRSS4 | Serine protease 4 | Serine protease 3 | TRY4 | Trypsin III | Trypsin IV | Trypsinogen 4 | MTG | Trypsinogen 5 | Pancreatic trypsinogen III | Protease | serine | 3 | Trypsin-3 | Mesotrypsinogen | PRSS3 | T9 | TRY3 | Trypsinogen IV
- Origin Species
- Human
- NCBI GenBank
- NM_002771
- SwissProt
- P35030
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 32.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MCGPDDRCPA RWPGPGRAVK CGKGLAAARP GRVERGGAQR GGAGLELHPL LGGRTWRAAR61DADGCEALGT VAVPFDDDDK IVGGYTCEEN SLPYQVSLNS GSHFCGGSLI SEQWVVSAAH121CYKTRIQVRL GEHNIKVLEG NEQFINAAKI IRHPKYNRDT LDNDIMLIKL SSPAVINARV181STISLPTAPP AAGTECLISG WGNTLSFGAD YPDELKCLDA PVLTQAECKA SYPGKITNSM241FCVGFLEGGK DSCQRDSGGP VVCNGQLQGV VSWGHGCAWK NRPGVYTKVY NYVDWIKDTI301AANSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
304 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.