Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PRSS21 / Testisin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106813-100
Catalog Number: LS-G106813
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRSS21 (NM_144957), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Eosinophil serine protease 1 | ESP1 | ESP-1 | Tryptase 4 | Serine protease 21 | TESP5 | TEST1 | Testisin | PRSS21
- Origin Species
- Human
- NCBI GenBank
- NM_006799
- SwissProt
- Q9Y6M0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 33.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGARGALLLA LLLARAGLRK PESQEAAPLS GPCGRRVITS RIVGGEDAEL GRWPWQGSLR61LWDSHVCGVS LLSHRWALTA AHCFETYSDL SDPSGWMVQF GQLTSMPSFW SLQAYYTRYF121VSNIYLSPRY LGNSPYDIAL VKLSAPVTYT KHIQPICLQA STFEFENRTD CWVTGWGYIK181EDEALPSPHT LQEVQVAIIN NSMCNHLFLK YSFRKDIFGD MGDSGGPLAC NKNGLWYQIG241VVSWGVGCGR PNRPGVYTNI SHHFEWIQKL MAQSGMSQPD PSWPLLFFPL LWALPLLGPVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
300 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.