Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PRR5L / FLJ14213 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109676-100
Catalog Number: LS-G109676
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FLJ14213(PRR5L) (NM_024841), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FLJ14213 | Proline rich 5 like | PROTOR-2 | PRR5L | Proline-rich protein 5-like | Protein observed with Rictor-2 | PROTOR2
- Origin Species
- Human
- NCBI GenBank
- NM_024841
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTRGFAPILP VEFHKMGSFR RPRPRFMSSP VLSDLPRFQA ARQALQLSSS SAWNSVQTAV61INVFKGGGLQ SNELYALNEN IRRLLKSELG SFITDYFQNQ LLAKGLFFVE EKIKLCEGEN121RIEVLAEVWD HFFTETLPTL QAIFYPVQGQ ELTIRQISLL GFRDLVLLKV KLGDLLLLAQ181SKLPSSIVQM LLILQSVHEP TGPSESYLQL EELVKQVVSP FLGISGDRSF SGPTYTLARR241HSRVRPKVTV LNYASPITAV SRPLNEMVLT PLTEQEGEAY LEKCGSVRRH TVANAHSDIQ301LLAMATMMHS GLGEEASSEN KCLLLPPSFP PPHRQCSSEP NITDNPDGLE EGARGSQEGS361ELNCASLSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
368 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.