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PRR5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PRR5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G129039-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRR5 (NM_181334), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Proline-rich protein 5 | PROTOR-1 | PROTOR1 | Protein observed with Rictor-1 | Proline rich 5 (renal) | PRR5 | PP610
Origin Species
Human
NCBI GenBank
NM_015366
SwissProt
P85299

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSPSLSDLG KREPAAAADE RGTQQRRACA NATWNSIHNG VIAVFQRKGL PDQELFSLNE
61GVRQLLKTEL GSFFTEYLQN QLLTKGMVIL RDKIRFYEEL QRDKAAAAAV LGAVRKRPSV
121VPMAGQDPAL STSHPFYDVA RHGILQVAGD DRFGRRVVTF SCCRMPPSHE LDHQRLLEYL
181KYTLDQYVEN DYTIVYFHYG LNSRNKPSLG WLQSAYKEFD RKYKKNLKAL YVVHPTSFIK
241VLWNILKPLI SHKFGKKVIY FNYLSELHEH LKYDQLVIPP EVLRYDEKLQ SLHEGRTPPP
301TKTPPPRPPL PTQQFGVSLQ YLKDKNQGEL IPPVLRFTVT YLREKGLRTE GLFRRSASVQ
361TVREIQRLYN QGKPVNFDDY GDIHIPAVIL KTFLRELPQP LLTFQAYEQI LGITCVESSL
421RVTGCRQILR SLPEHNYVVL RYLMGFLHAV SRESIFNKMN SSNLACVFGL NLIWPSQGVS
481SLSALVPLNM FTELLIEYYE KIFSTPEAPG EHGLAPWEQG SRAAPLQEAV PRTQATGLTK
541PTLPPSPLMA ARRRL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

555 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.