Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PRR35 / C16orf11 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106710-100
Catalog Number: LS-G106710
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRR35 (NM_145270), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C16orf11 | LA16c-366D1.2 | Uncharacterized protein RJD1
- Origin Species
- Human
- NCBI GenBank
- NM_145270
- SwissProt
- P0CG20
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSREAGSCRV GTGARARSRK PKKPHYIPRP WGKPYNYKCF QCPFTCLEKS HLYNHMKYSL61CKDSLSLLLD SPDWACRRGS TTPRPHAPTP DRPGESDPGR QPQGARPTGA APAPDLVVAD121IHSLHCGGGP KSRAKGSPGP PPPVARATRK GPGPSGLLPE SWKPGMGGDP RGVGAGDMAS181AGPEGSVPCY PPPAPGEFPE AHSLHLSLLG VNYPLSPGLF SYLGPSLAAA AHVPFLASAS241PLLPPATAFP AVQPPQRPTP APRLYYPLLL EHTLGLPAGK AALAKAPVSP RSPSGTPAPG301LLKVPVPGLG PWPRVTPRDP GQEGELERAA QSDPRRRLSL GSRLELPKAS PSLTRFCSRS361SLPTGSSVML WPEDGDPGGP ETPGPEGPLP LQPRGPVPGS PEHVGEDLTR ALGDYARVEQ421RLGQLGPAGG LAPRPLREQL GKIRLELLTI HQALEQAVRP PDAPLDLSVK RAPAKGPQAL481GEAWGRPELG PVLTGGTPEP PGMLGPAAPQ PFSGHTTKCE ADSSVPPPGL PLAAPDDPVI541PGSGWGTCVA TRSSQTPEAV CGLQSPQGAE VTRTRPLEQKLISEEDLAANDILDYKDDDDKV
571 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.