LSBio
PRR19 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PRR19 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PRR19 antibodies →
$504.00each
Catalog Number: LS-G103375-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRR19 (NM_199285), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PRR19 | Proline rich 19 | Proline-rich protein 19
Origin Species
Human
NCBI GenBank
AI871282
SwissProt
A6NJB7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
38.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MDTQGPVSQP FQQPEKPGRV RRRKTRRERN KALVGSRRPL AHHDPPVAIR DPPVVPTASK
61LVVITQGRLS REHRGLFNHE VKSLDVARLL SSGTLVPGSP TLPAKPSPSP GRAQEPAPRS
121RDKENQVPGG SGPGPPSSPE LSGVGQLLAE LQCQLSLPQA FPRRNLIQDA RDAIVHTLQA
181CHGCVPDLAL VLRGCQPPLP GAKPGVSERK MTPFWINSPD QVPEQERQRK QQGTKEFTFP
241MPYTSSMPTA HRGSLAPPRG PWPPYFPSLS SPSGTAWGPP TAFDLLKSIW LVATPPPPRP
301WGVGLPQPLP QPSSPLLPRT SVLDWSPSPP SPLPSLSWVV AQSSPEAWSF PPMRLY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

356 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.