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PRR14 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PRR14 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PRR14 antibodies →
$504.00each
Catalog Number: LS-G110096-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRR14 (NM_024031), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Proline-rich protein 14 | PRR14 | Proline rich 14
Origin Species
Human
NCBI GenBank
NM_024031
SwissProt
Q9BWN1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDLPGDSSPP GQPRLCRQPL TRALWGARSP KRPRLQLPGA PSPLEKASRR VLAVVLEDVM
61AVHMVPVVPS KQTSIPQHHS YHQDPVHRQP PASPPRQAGW SSQARPPDPL CLCREPLSRI
121HRTSSTLRRR SRTTPGPEEG PSQKVDRAPQ PTLVVMLEDI ASPRPPAEGF IDETPNFIIP
181AQRAEPMRIV RQPTPPPGDL EPPFQPSALP ADPLESPPTA PDPALELPST PPPSSLLRPR
241LSPWGLAPLF RSVRSKLESF ADIFLTPNKT PQPPPPSPPM KLELKIAISE AEQSGAAEGT
301ASVSPRPPIR QWRTQDHNTP ALLPKPSLGR SYSCPDLGPP GPGTCTWPPA PPQPSRPRPR
361RHTVGGGEMA RAPPPPRPCL RKEVFPLGGV GASPSLTTSC SSTASTSFSE PAEPRLGSTK
421GKEPRASKDQ VLSEPETKTM GKVSRFRIRR TPARPQLNLT PMGLPRPIRL NKKEFSLEEI
481YTNKNYQSPT TRRTFETIFE EPRERNGTLI FTSSRKLRRA VEFRDSSLPR SRRPSRGVRA
541AGGRTVPPNV APSPDVGPLL QQRLEELDAL LLEEETVDRE QPHWT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

585 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.