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PRPF40B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PRPF40B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PRPF40B antibodies →
$504.00each
Catalog Number: LS-G120702-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRPF40B (NM_001031698), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Huntingtin yeast partner C | PRPF40B | HYPC
Origin Species
Human
NCBI GenBank
T29975

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMPPPFMPPP GIPPPFPPMG LPPMSQRPPA IPPMPPGILP PMLPPMGAPP PLTQIPGMVP
61PMMPGMLMPA VPVTAATAPG ADTASSAVAG TGPPRALWSE HVAPDGRIYY YNADDKQSVW
121EKPSVLKSKA ELLLSQCPWK EYKSDTGKPY YYNNQSKESR WTRPKDLDDL EVLVKQEAAG
181KQQQQLPQTL QPQPPQPQPD PPPVPPGPTP VPTGLLEPEP GGSEDCDVLE ATQPLEQGFL
241QQLEEGPSSS GQHQPQQEEE ESKPEPERSG LSWSNREKAK QAFKELLRDK AVPSNASWEQ
301AMKMVVTDPR YSALPKLSEK KQAFNAYKAQ REKEEKEEAR LRAKEAKQTL QHFLEQHERM
361TSTTRYRRAE QTFGELEVWA VVPERDRKEV YDDVLFFLAK KEKEQAKQLR RRNIQALKSI
421LDGMSSVNFQ TTWSQAQQYL MDNPSFAQDH QLQNMDKEDA LICFEEHIRA LEREEEEERE
481RARLRERRQQ RKNREAFQTF LDELHETGQL HSMSTWMELY PAVSTDVRFA NMLGQPGSTP
541LDLFKFYVEE LKARFHDEKK IIKDILKDRG FCVEVNTAFE DFAHVISFDK RAAALDAGNI
601KLTFNSLLEK AEAREREREK EEARRMRRRE AAFRSMLRQA VPALELGTAW EEVRERFVCD
661SAFEQITLES ERIRLFREFL QVLEQTECQH LHTKGRKHGR KGKKHHHKRS HSPSGSESEE
721EELPPPSLRP PKRRRRNPSE SGSEPSSSLD SVESGGAALG GRGSPSSHLL GADHGLRKAK
781KPKKKTKKRR HKSNSPESET DPEEKAGKES DEKEQEQDKD RELQQAELPN RSPGFGIKKE
841KTGWDTSESE LSEGELERRR RTLLQQLDDH Q
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

871 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.