LSBio
PROX2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PROX2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PROX2 antibodies →
$725.00each
Catalog Number: LS-G120196-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PROX2 (NM_001080408), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Prospero homeobox protein 2 | Prospero homeobox 2 | PROX-2 | PROX2
Origin Species
Human
SwissProt
Q3B8N5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDPNSILLSP QPQICSHLAE ACTEGERSSS PPELDRDSPF PWSQVPSSSP TDPEWFGDEH
61IQAKRARVET IVRGMCLSPN PLVPGNAQAG VSPRCPKKAR ERKRKQNLPT PQGLLMPAPA
121WDQGNRKGGP RVREQLHLLK QQLRHLQEHI LQAAKPRDTA QGPGGCGTGK GPLSAKQGNG
181CGPRPWVVDG DHQQGTSKDL SGAEKHQESE KPSFLPSGAP ASLEILRKEL TRAVSQAVDS
241VLQKVLLDPP GHLTQLGRSF QGQVAEGRSE PSPPVGGACK DPLALAALPR RVQLQAGVPV
301GNLSLAKRLD SPRYPIPPRM TPKPCQDPPA NFPLTAPSHI QENQILSQLL GHRYNNGHWS
361SSPPQDSSSQ RHPSSEPALR PWRTTKPQPL VLSQQQCPLP FTSAHLESLP LLPSVKMEQR
421GLHAVMEALP FSLLHIQEGL NPGHLKKAKL MFFFTRYPSS NLLKVYFPDV QFNRCITSQM
481IKWFSNFREF YYIQMEKSAR QAISDGVTNP KMLVVLRNSE LFQALNMHYN KGNDFEVPDC
541FLEIASLTLQ EFFRAVSAGR DSDPSWKKPI YKIISKLDSD IPEIFKSSSY PQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

592 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.