LSBio
Prominin 2 / PROM2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Prominin 2 / PROM2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PROM2 antibodies →
$725.00each
Catalog Number: LS-G102290-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Prominin 2(PROM2) (NM_144707), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HPROML2 | PROML2 | Prominin-like protein 2 | Prominin-related protein | PROM-2 | PROM2 | Prominin 2 | Prominin-2
Origin Species
Human
NCBI GenBank
NM_144707
SwissProt
Q8N271

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
91.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKHTLALLAP LLGLGLGLAL SQLAAGATDC KFLGPAEHLT FTPAARARWL APRVRAPGLL
61DSLYGTVRRF LSVVQLNPFP SELVKALLNE LASVKVNEVV RYEAGYVVCA VIAGLYLLLV
121PTAGLCFCCC RCHRRCGGRV KTEHKALACE RAALMVFLLL TTLLLLIGVV CAFVTNQRTH
181EQMGPSIEAM PETLLSLWGL VSDVPQELQA VAQQFSLPQE QVSEELDGVG VSIGSAIHTQ
241LRSSVYPLLA AVGSLGQVLQ VSVHHLQTLN ATVVELQAGQ QDLEPAIREH RDRLLELLQE
301ARCQGDCAGA LSWARTLELG ADFSQVPSVD HVLHQLKGVP EANFSSMVQE ENSTFNALPA
361LAAMQTSSVV QELKKAVAQQ PEGVRTLAEG FPGLEAASRW AQALQEVEES SRPYLQEVQR
421YETYRWIVGC VLCSVVLFVV LCNLLGLNLG IWGLSARDDP SHPEAKGEAG ARFLMAGVGL
481SFLFAAPLIL LVFATFLVGG NVQTLVCRSW ENGELFEFAD TPGNLPPSMN LSQLLGLRKN
541ISIHQAYQQC KEGAALWTVL QLNDSYDLEE HLDINQYTNK LRQELQSLKV DTQSLDLLSS
601AARRDLEALQ SSGLQRIHYP DFLVQIQRPV VKTSMEQLAQ ELQGLAQAQD NSVLGQRLQE
661EAQGLRNLHQ EKVVPQQSLV AKLNLSVRAL ESSAPNLQLE TSDVLANVTY LKGELPAWAA
721RILRNVSECF LAREMGYFSQ YVAWVREEVT QRIATCQPLS GALDNSRVIL CDMMADPWNA
781FWFCLAWCTF FLIPSIIFAV KTSKYFRPIR KRLSSTSSEE TQLFHIPRVT SLKL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

834 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.