Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PRODH2 / HSPOX1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G101742-100
Catalog Number: LS-G101742
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRODH2 (NM_021232), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Probable proline oxidase 2 | SCZD4 | PRODH2 | HSPOX1
- Origin Species
- Human
- NCBI GenBank
- NM_021232
- SwissProt
- Q9UF12
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSPRVVSNSS VLASQSVGIT NVRTVFSNVF NNTTAFPILR GSNCHKITAP GLGKGQLVNL61LPPENLPWCG GSQGPRMLRT CYVLCSQAGP PSRGWQSLSF DGGAFHLKGT GELTRALLVL121RLCAWPPLVT HGLLLQAWSR RLLGSRLSGA FLRASVYGQF VAGETAEEVK GCVQQLRTLS181LRPLLAVPTE EEPDSAAKSG EAWYEGNLGA MLRCVDLSRG LLEPPSLAEA SLMQLKVTAL241TSTRLCKELA SWVRRPGASL ELSPERLAEA MDSGQNLQVS CLNAEQNQHL RASLSRLHRV301AQYARAQHVR LLVDAEYTSL NPALSLLVAA LAVRWNSPGE GGPWVWNTYQ ACLKDTFERL361GRDAEAAHRA GLAFGVKLVR GAYLDKERAV AQLHGMEDPT QPDYEATSQS YSRCLELMLT421HVARHGPMCH LMVASHNEES VRQATKRMWE LGIPLDGTVC FGQLLGMCDH VSLALGQAGY481VVYKSIPYGS LEEVIPYLIR RAQENRSVLQ GARREQELLS QELWRRLLPG CRRIPHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
536 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.