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PROB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PROB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PROB1 antibodies →
$725.00each
Catalog Number: LS-G128407-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PROB1 (NM_001161546), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001161546

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
106.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLTALAPPAL PGIPRQLPTA PARRQDSSGS SGSYYTAPGS PEPPDVGPDA KGPANWPWVA
61PGRGAGAQPR LSVSAQNSRQ RHGPGSGFPR GPGSGPRPPQ PQLRTLPSGE MEVIFGVGPL
121FGCSGADDRE AQQQFTEPAF ISPLPPGPAS PAAVPRQSQV PDGGSRWATY LELRPRGPSP
181AAPAQFECVE VALEEGAAPA RPRTVPKRQI ELRPRPQSPP RAAGAPRPRL LLRTGSLDES
241LGPLQAAAGF VQTALARKLS PEAPAPSSAT FGSTGRSEPE TRETARSTHV VLEKAKSRPL
301RVRDNSAPAK APRPWPSLRE RAIRRDKPAP GTEPLGPVSS SIFLQSEEKI QEARKTRFPR
361EAPDRTVQRA RSPPFECRIP SEVPSRAVRP RSPSPPRQTP NGAVRGPRCP SPQNLSPWDR
421TTRRVSSPLF PEASSEWENQ NPAVEETVSR RSPSPPILSQ WNQCVAGERS PSLEAPSLWE
481IPHSAVADAV EPRSSPSPPA FFPWEAPDRP IGTWGPSPQE TWDPMGPGSS IAFTQEAQNG
541LTQEELAPPT PSAPGTPEPT EMQSPSTREI SDLAFGGSQQ SPEVAAPEPP GSHPVGTLDA
601DKCPEVLGPG EAASGRPRMA IPRPRDVRKL VKTTYAPGFP AGAQGSGLPA PPADPCGEEG
661GESKTQEPPA LGPPAPAHYT SVFIKDFLPV VPHPYEPPEP SFDTVARDAS QPNGVLRRRA
721ENSTAKPFKR TEIRLPGALA LGRRPEVTSR VRARGPGGEN RDVEAQRLVP DGDGRTSPLG
781GARSSSQRSP VGPAGVRSPR PGSPQMQASP SPGIAPKPKT PPTAPEPAAA VQAPLPREPL
841ALAGRTAPAQ PRAASAPPTD RSPQSPSQGA RRQPGAAPLG KVLVDPESGR YYFVEAPRQP
901RLRVLFDPES GQYVEVLLPP SSPGPPHRVY TPLALGLGLY PPAYGPIPSL SLPPSPGPQA
961LGSPQLPWVS EAGPLDGTYY LPVSGTPNPA PPLLLCAPPS SSGPTQPGKG SLFPL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1015 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.