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PRKY Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PRKY Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PRKY antibodies →
$504.00each
Catalog Number: LS-G99860-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Protein kinase Y linked(PRKY) (NM_002760), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PRKY | PRKXP3 | PRKYP | Protein kinase | Y-linked | Protein kinase Y linked
NCBI GenBank
NM_002760
SwissProt
O43930

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
31.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MEAPGPAQAA AAESNSREVT EDAADWAPAL CPSPEARSPE APAYRLQDCD ALVTMGTGTF
61GRVHLVKEKT AKHFFALKVM SIPDVIRRKQ EQHVHNEKSV LKEVSHPFLI RLFWTWHEER
121FLYMLMEYVP GGELFSYLRN RGHFSSTTGL FYSAEIICAI EYLHSKEIVY RDLKPENILL
181DRDGHIKLTD FGFAKKLVDR TWTLCGTPEY LAPEVIQSKG HGRAVDWWAL GILIFEMLSG
241FPPFFDDNPF GIYQKILAGK LYFPRHLDFH VKTGRMM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

277 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.