Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PRKAA1 / AMPK Alpha 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102944-100
Catalog Number: LS-G102944
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AMPK alpha 1(PRKAA1) (NM_206907), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ACACA kinase | AMPK alpha 1 | AMPK alpha-1 chain | Acetyl-CoA carboxylase kinase | AMPKa1 | AMPK subunit alpha-1 | AMPK | AMPK1 | Tau-protein kinase PRKAA1 | HMGCR kinase | PRKAA1
- Origin Species
- Human
- NCBI GenBank
- NM_006251
- SwissProt
- Q13131
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 65.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MRRLSSWRKM ATAEKQKHDG RVKIGHYILG DTLGVGTFGK VKVGKHELTG HKVAVKILNR61QKIRSLDVVG KIRREIQNLK LFRHPHIIKL YQVISTPSDI FMVMEYVSGG ELFDYICKNG121RKSDVPGVVK TGSTKELDEK ESRRLFQQIL SGVDYCHRHM VVHRDLKPEN VLLDAHMNAK181IADFGLSNMM SDGEFLRTSC GSPNYAAPEV ISGRLYAGPE VDIWSSGVIL YALLCGTLPF241DDDHVPTLFK KICDGIFYTP QYLNPSVISL LKHMLQVDPM KRATIKDIRE HEWFKQDLPK301YLFPEDPSYS STMIDDEALK EVCEKFECSE EEVLSCLYNR NHQDPLAVAY HLIIDNRRIM361NEAKDFYLAT SPPDSFLDDH HLTRPHPERV PFLVAETPRA RHTLDELNPQ KSKHQGVRKA421KWHLGIRSQS RPNDIMAEVC RAIKQLDYEW KVVNPYYLRV RRKNPVTSTY SKMSLQLYQV481DSRTYLLDFR SIDDEITEAK SGTATPQRSG SVSNYRSCQR SDSDAEAQGK SSEVSLTSSV541TSLDSSPVDL TPRPGSHTIE FFEMCANLIK ILAQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
574 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.