Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PRIMPOL / CCDC111 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106087-100
Catalog Number: LS-G106087
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Coiled coil domain containing protein 111(PRIMPOL) (NM_152683), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_152683
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNRKWEAKLK QIEERASHYE RKPLSSVYRP RLSKPEEPPS IWRLFHRQAQ AFNFVKSCKE61DVHVFALECK VGDGQRIYLV TTYAEFWFYY KSRKNLLHCY EVIPENAVCK LYFDLEFNKP121ANPGADGKKM VALLIEYVCK ALQELYGVNC SAEDVLNLDS STDEKFSRHL IFQLHDVAFK181DNIHVGNFLR KILQPALDLL GSEDDDSAPE TTGHGFPHFS EAPARQGFSF NKMFTEKATE241ESWTSNSKKL ERLGSAEQSS PDLSFLVVKN NMGEKHLFVD LGVYTRNRNF RLYKSSKIGK301RVALEVTEDN KFFPIQSKDV SDEYQYFLSS LVSNVRFSDT LRILTCEPSQ NKQKGVGYFN361SIGTSVETIE GFQCSPYPEV DHFVLSLVNK DGIKGGIRRW NYFFPEELLV YDICKYRWCE421NIGRAHKSNN IMILVDLKNE VWYQKCHDPV CKAENFKSDC FPLPAEVCLL FLFKEEEEFT481TDEADETRSN ETQNPHKPSP SRLSTGASAD AVWDNGIDDA YFLEATEDAE LAEAAENSLL541SYNSEVDEIP DELIIEVLQETRTRPLEQKLISEEDLAANDILDYKDDDDKV
560 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.