Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PRDX6 / Peroxiredoxin 6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100414-100
Catalog Number: LS-G100414
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Peroxiredoxin 6(PRDX6) (NM_004905), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 24 kDa protein | 1-Cys | 1-Cys peroxiredoxin | 1-Cys PRX | AOP2 | Antioxidant protein 2 | AiPLA2 | KIAA0106 | Liver 2D page spot 40 | NSGPx | p29 | PRDX6 | Peroxiredoxin 6 | Peroxiredoxin-6 | Red blood cells page spot 12
- Origin Species
- Human
- NCBI GenBank
- NM_004905
- SwissProt
- P30041
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 24.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPGGLLLGDV APNFEANTTV GRIRFHDFLG DSWGILFSHP RDFTPVCTTE LGRAAKLAPE61FAKRNVKLIA LSIDSVEDHL AWSKDINAYN CEEPTEKLPF PIIDDRNREL AILLGMLDPA121EKDEKGMPVT ARVVFVFGPD KKLKLSILYP ATTGRNFDEI LRVVISLQLT AEKRVATPVD181WKDGDSVMVL PTIPEEEAKK LFPKGVFTKE LPSGKKYLRY TPQPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
224 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.