Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PRDX3 / Peroxiredoxin 3 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114146-100
Catalog Number: LS-G114146
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Peroxiredoxin 3(PRDX3) (NM_014098), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Antioxidant protein 1 | AOP1 | AOP-1 | HBC189 | Peroxiredoxin III | PRDX3 | PRO1748 | Peroxiredoxin-3 | Prx-III | SP-22 | Peroxiredoxin 3 | Protein MER5 homolog
- Origin Species
- Human
- NCBI GenBank
- NM_006793
- SwissProt
- P30048
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 25.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAAVGRLLR ASVARHVSAI PWGISATAAL RPAACGRTSL TNLLCSGSSQ APYFKGTAVV61NGEFKDLSLD DFKGKYLVLF FYPLDFTFVC PTEIVAFSDK ANEFHDVNCE VVAVSVDSHF121SHLAWINTPR KNGGLGHMNI ALLSDLTKQI SRDYGVLLEG SGLALRGLFI IDPNGVIKHL181SVNDLPVGRS VEETLRLVKA FQYVETHGEV CPANWTPDSP TIKPSPAASK EYFQKVNQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
238 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.