LSBio
PRDM15 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PRDM15 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PRDM15 antibodies →
$725.00each
Catalog Number: LS-G110474-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRDM15 (NM_022115), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C21orf83 | PFM15 | PR domain containing 15 | Zinc finger protein 298 | ZNF298 | PRDM15
Origin Species
Human
NCBI GenBank
AF276513
SwissProt
P57071

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
169.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPRRRPPASG AAQFPERIAT RSPDPIPLCT FQRXPRAAPV QPPCRLFFVT FAGCGHRWRS
61ESKPGWISRS RSGIALRAAR PPGSSPPRPA APRPPPPGGV VAEAPGDVVI PRPRVQPMRV
121ARGGPWTPNP AFREAESWSQ IGNQRVSEQL LETSLGNEVS DTEPLSPASA GLRRNPALPP
181GPFAQNFSWG NQENLPPALG KIANGGGTGA GKAECGYETE SHLLEPHEIP LNVNTHKFSD
241CEFPYEFCTV CFSPFKLLGM SGVEGVWNQH SRSASMHTFL NHSATGIREA GCRKDMXVSE
301MAEDGSEEIM FIWCEDCSQY HDSECPELGP VVMVKDSFVL SRARSWPASG HVHTQAGQGM
361RGYEDRDRAD PQQLPEAVPA GLVRRLSGQQ LPCRSTLTWG RLCHLVAQGR SSLPPNLEIR
421RLEDGAEGVF AITQLVKRTQ FGPFESRRVA KWEKESAFPL KVFQKDGHPV CFDTSNEDDC
481NWMMLVRPAA EAEHQNLTAY QHGSDVYFTT SRDIPPGTEL RVWYAAFYAK KMDKPMLKQA
541GSGVHAAGTP ENSAPVESEP SQWACKVCSA TFLELQLLNE HLLGHLEQAK SLPPGSQSEA
601AAPEKEQDTP RGEPPAVPES ENVATKEQKK KPRRGRKPKV SKAEQPLVIV EDKEPTEQVA
661EIITEVPPDE PVSATPDERI MELVLGKLAT TTTDTSSVPK FTHHQNNTIT LKRSLILSSR
721HGIRRKLIKQ LGEHKRVYQC NICSKIFQNS SNLSRHVRSH GDKLFKCEEC AKLFSRKESL
781KQHVSYKHSR NEVDGEYRYR CGTCEKTFRI ESALEFHNCR TDDKTFQCEM CFRFFSTNSN
841LSKHKKKHGD KKFACEVCSK MFYRKDVMLD HQRRHLEGVR RVKREDLEAG GENLVRYKKE
901PSGCPVCGKV FSCRSNMNKH LLTHXDKKYT CEICGRKFFR VDVLRDHIHV HFKDIALMDD
961HQREEFIGKI GISSEENDDN SDESADSEPH KYSCKRCQLT FGRGKEYLKH IMEVHKEKGX
1021GCSICNRRFA LXATYHAHMV IHRENLPDPN VQKYIHPCEI CGRIFNSIGN LERHKLIHTG
1081VKSHACEQCG KSFARKDMLK EHMRVHDNVR EYLCAECGKG MKTKHALRHH MKLHKGIKEY
1141ECKECHRRFA QKVNMLKHCK RHTGIKDFMC ELCGKTFSER NTMETHKLIH TVGKQWTCSV
1201CDKKYVTEYM LQKHVQLTHD KVEAQSCQLC GTKVSTRASM SRHMRRKHPE VLAVRIDDLD
1261HLPETTTIDA SSIGIVQPEL TLEQEDLAEG KHGKAAKRSH KRKQKPEEEA GAPVPEDATF
1321SEYSEKETEF TGSVGDETNS AVQSIQQVVV TLGDPNVTTP SSSVGLTNIT VTPITTAAAT
1381QFTNLQPVAV GHLTTPERQL QLDNSILTVT FDTVSGSAML HNRQNDVQIH PQPEASNPQS
1441VAHFINLTTL VNSITPLGSQ LSDQHPLTWR AVPQTDVLPP SQPQAPPQQA AQPQVQAEQQ
1501QQQMYSY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1507 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.