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PRDM12 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PRDM12 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PRDM12 antibodies →
$504.00each
Catalog Number: LS-G110658-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRDM12 (NM_021619), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PRDM12 | PR domain containing 12 | PFM9
Origin Species
Human
NCBI GenBank
NM_021619
SwissProt
Q9H4Q4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
40.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMGSVLPAEA LVLKTGLKAP GLALAEVITS DILHSFLYGR WRNVLGEQLF EDKSHHASPK
61TAFTAEVLAQ SFSGEVQKLS SLVLPAEVII AQSSIPGEGL GIFSKTWIKA GTEMGPFTGR
121VIAPEHVDIC KNNNLMWEVF NEDGTVRYFI DASQEDHRSW MTYIKCARNE QEQNLEVVQI
181GTSIFYKAIE MIPPDQELLV WYGNSHNTFL GIPGVPGLEE DQKKNKHEDF HPADSAAGPA
241GRMRCVICHR GFNSRSNLRS HMRIHTLDKP FVCRFCNRRF SQSSTLRNHV RLHTGERPYK
301CQVCQSAYSQ LAGLRAHQKS ARHRPPSTAL QAHSPALPAP HAHAPALAAA AAAAAAAAAH
361HLPAMVL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

367 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.