LSBio
PRDM11 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PRDM11 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PRDM11 antibodies →
$725.00each
Catalog Number: LS-G111271-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRDM11 (NM_020229), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PFM8 | PRDM11 | PR domain containing 11
Origin Species
Human
NCBI GenBank
NM_020229
SwissProt
Q9NQV5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLKMAEPIAS LMIVECRACL RCSPLFLYQR EKDRMTENMK ECLAQTNAAV GDMVTVVKTE
61VCSPLRDQEY GQPCSRRPDS SAMEVEPKKL KGKRDLIVPK SFQQVDFWFC ESCQEYFVDE
121CPNHGPPVFV SDTPVPVGIP DRAALTIPQG MEVVKDTSGE SDVRCVNEVI PKGHIFGPYE
181GQISTQDKSA GFFSWLIVDK NNRYKSIDGS DETKANWMRY VVISREEREQ NLLAFQHSER
241IYFRACRDIR PGEWLRVWYS EDYMKRLHSM SQETIHRNLA RGEKRLQREK SEQVLDNPED
301LRGPIHLSVL RQGKSPYKRG FDEGDVHPQA KKKKIDLIFK DVLEASLESA KVEAHQLALS
361TSLVIRKVPK YQDDAYSQCA TTMTHGVQNI GQTQGEGDWK VPQGVSKEPG QLEDEEEEPS
421SFKADSPAEA SLASDPHELP TTSFCPNCIR LKKKVRELQA ELDMLKSGKL PEPPVLPPQV
481LELPEFSDPA GKLVWMRLLS EGRVRSGLCG G
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

511 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.