Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PRCC / RCCP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115619-100
Catalog Number: LS-G115619
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Papillary renal cell carcinoma(PRCC) (NM_005973), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Proline-rich protein PRCC | PRCC | RCCP1 | TPRC
- Origin Species
- Human
- NCBI GenBank
- NM_005973
- SwissProt
- Q92733
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSLVAYASSD ESEPDEAEPE PEEEEAVAPT SGPALGGLFA SLPAPKGPAL LPPPPQMLAP61AFPPPLLLPP PTGDPRLQPP PPLPFGLGGF PPPPGVSPAE AAGVGEGLGL GLPSPRGPGL121NLPPPIGGAG PPLGLPKPKK RKEPVKIAAP ELHKGDSDSE EDEPTKKKTI LQGSSEGTGL181SALLPQPKNL TVKETNRLLL PHAFSRKPSD GSPDTKPSRL ASKTKTSSLA PVVGTTTTTP241SPSAIKAAAK SAALQVTKQI TQEEDDSDEE VAPENFFSLP EKAEPPGVEP YPYPIPTVPE301ELPPGTEPEP AFQDDAANAP LEFKMAAGSS GAPWMPKPGD DYSYNQFSTY GDANAAGAYY361QDYYSGGYYP AQDPALVPTQ EIAPDASFID DEAFKRLQGK RNRGREEINF VEIKGDDQLS421GAQQWMTKSL TEEKTMKSFS KKKGEQPTGQ QRRKHQITYL IHQAKERELE LKNTWSENKL481SRRQTQAKYG FTRTRPLEQKLISEEDLAANDILDYKDDDDKV
491 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.