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PRAMEF3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PRAMEF3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PRAMEF3 antibodies →
$725.00each
Catalog Number: LS-G121535-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRAMEF3 (NM_001013692), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PRAMEF3 | PRAME family member 3
Origin Species
Human
NCBI GenBank
NM_001013692
SwissProt
Q5TYW8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MRMSLQAPRR LLELAGQSLL GDQALAISIL DELPRELFPP LFVEAFTSRR CEVLKVMVQA
61WPFPCLPLGS LMKTPDLEIL HYVVDGIDCL LAQKVRPRRR KLQVLELRDV DENFWTIWSG
121ARPLSCSPEA MSKRQTVEDC PRTGEKQPLK VFMDVCLKEK FMDEDLSFFS GWVQHRRGSV
181HLCCTKVVNY SMSILNFRNI LETVYPDSIQ VLEIWNMCWP CMIVEFIRYL SQMRNLRKLF
241ISDGCRYLLS SDSQEQLVAE FSSVLLRLEY LQMLYVRRVC FFRGHLDQLI RCLRSPLETL
301ALTYGFLEKV DLKCLPRYPS LSQLKQLNLS HGALRFIRLE PLRALLEKVA ATLQTLFLVD
361CGIRDSKLRV ILPALSCCSN LTTFCFHGND TSMDGLKDLL RHTGRLSNLS LETYPAPRES
421LDDRGRVISE LLTPLQAELM RILREVREPK RIFFGPVSCP CCGTSPTEQL EFNFCLWGRP
481A
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

481 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.