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PRAM1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PRAM1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PRAM1 antibodies →
$504.00each
Catalog Number: LS-G109001-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRAM1 (NM_032152), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PML-RAR | PRAM1 | PRAM | PRAM-1
Origin Species
Human
NCBI GenBank
NM_032152
SwissProt
Q96QH2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAHHLPAAME NHQDFRSIKA KFQASQPEPS DLPKKPPKPE FGKLKKFSQP ELSEHPKKAP
61LPEFGAVSLK PPPPEVTDLP KKPPPPEVTD LPKKPPPPEV TDLPKKPPPP EVTDLPKKPS
121KLELSDLSKK FPQLGATPFP RKPLQPEVGE APLKASLPEP GAPARKPLQP DELSHPARPP
181SEPKSGAFPR KLWQPEAGEA TPRSPQPELS TFPKKPAQPE FNVYPKKPPQ PQVGGLPKKS
241VPQPEFSEAA QTPLWKPQSS EPKRDSSAFP KKASQPPLSD FPKKPPQPEL GDLTRTSSEP
301EVSVLPKRPR PAEFKALSKK PPQPELGGLP RTSSEPEFNS LPRKLLQPER RGPPRKFSQP
361EPSAVLKRHP QPEFFGDLPR KPPLPSSASE SSLPAAVAGF SSRHPLSPGF GAAGTPRWRS
421GGLVHSGGAR PGLRPSHPPR RRPLPPASSL GHPPAKPPLP PGPVDMQSFR RPSAASIDLR
481RTRSAAGLHF QDRQPEDIPQ VPDEIYELYD DVEPRDDSSP SPKGRDEAPS VQQAARRPPQ
541DPALRKEKDP QPQQLPPMDP KLLKQLRKAE KAEREFRKKF KFEGEIVVHT KMMIDPNAKT
601RRGGGKHLGI RRGEILEVIE FTSNEEMLCR DPKGKYGYVP RTALLPLETE VYDDVDFCDP
661LENQPLPLGR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

670 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.