Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PPP6R3 / SAPS3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G128382-100
Catalog Number: LS-G128382
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SAPS3(PPP6R3) (NM_001164160), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C11orf23 | KIAA1558 | SAPL | SAPS domain family | member 3 | SAPLa | SAPS3 | PP6R3 | PPP6R3 | SAP190 | SAPS domain family member 3
- Origin Species
- Human
- NCBI GenBank
- NM_018312
- SwissProt
- Q5H9R7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 98.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFWKFDLHSS SHIDTLLERE DVTLKELMDE EDVLQECKAQ NRKLIEFLLK AECLEDLVSF61IIEEPPQDMD EKIRYKYPNI SCELLTSDVS QMNDRLGEDE SLLMKLYSFL LNDSPLNPLL121ASFFSKVLSI LISRKPEQIV DFLKKKHDFV DLIIKHIGTS AIMDLLLRLL TCIEPPQPRQ181DVLNWLNEEK IIQRLVEIVH PSQEEDRHSN ASQSLCEIVR LSRDQMLQIQ NSTEPDPLLA241TLEKQEIIEQ LLSNIFHKEK NESAIVSAIQ ILLTLLETRR PTFEGHIEIC PPGMSHSACS301VNKSVLEAIR GRLGSFHELL LEPPKKSVMK TTWGVLDPPV GNTRLNVIRL ISSLLQTNTS361SINGDLMELN SIGVILNMFF KYTWNNFLHT QVEICIALIL ASPFENTENA TITDQDSTGD421NLLLKHLFQK CQLIERILEA WEMNEKKQAE GGRRHGYMGH LTRIANCIVH STDKGPNSAL481VQQLIKDLPD EVRERWETFC TSSLGETNKR NTVDLVTTCH IHSSSDDEID FKETGFSQDS541SLQQAFSDYQ MQQMTSNFID QFGFNDEKFA DQDDIGNVSF DRVSDINFTL NTNESGNIAL601FEACCKERIQ QFDDGGSDEE DIWEEKHIAF TPESQRRSSS GSTDSEESTD SEEEDGAKQD661LFEPSSANTE DKMEVDLSEP PNWSANFDVP METTHGAPLD SVGSDVWSTE EPMPTKETGW721ASFSEFTSSL STKDSLRSNS PVEMETSTEP MDPLTPSAAA LAVQPEAAGS VAMEASSDGE781EDAESTDKVT ETVMNGGMKE TLSLTVDAKT ETAVFKRVLK SYREEGKLST SQDAACKDAE841ECPETAEAKC AAPRPPSSSP EQRTGQPSAP GDTSVNGPVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
879 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.