Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PPP6R2 / SAPS2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113798-100
Catalog Number: LS-G113798
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SAPS2(PPP6R2) (NM_014678), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DJ579N16.1 | SAPS domain family member 2 | SAPS2 | PPP6R2 | KIAA0685 | PP6R2 | SAP190 | SAPS domain family | member 2
- Origin Species
- Human
- NCBI GenBank
- NM_014678
- SwissProt
- O75170
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 101.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFWKFDLNTT SHVDKLLDKE HVTLQELMDE DDILQECKAQ NQKLLDFLCR QQCMEELVSL61ITQDPPLDME EKVRFKYPNT ACELLTCDVP QISDRLGGDE SLLSLLYDFL DHEPPLNPLL121ASFFSKTIGN LIARKTEQVI TFLKKKDKFI SLVLKHIGTS ALMDLLLRLV SCVEPAGLRQ181DVLHWLNEEK VIQRLVELIH PSQDEDRQSN ASQTLCDIVR LGRDQGSQLQ EALEPDPLLT241ALESRQDCVE QLLKNMFDGD RTESCLVSGT QVLLTLLETR RVGTEGLVDS FSQGLERSYA301VSSSVLHGIE PRLKDFHQLL LNPPKKKAIL TTIGVLEEPL GNARLHGARL MAALLHTNTP361SINQELCRLN TMDLLLDLFF KYTWNNFLHF QVELCIAAIL SHAAREERTE ASGSESRVEP421PHENGNRSLE TPQPAASLPD NTMVTHLFQK CCLVQRILEA WEANDHTQAA GGMRRGNMGH481LTRIANAVVQ NLERGPVQTH ISEVIRGLPA DCRGRWESFV EETLTETNRR NTVDLAFSDY541QIQQMTANFV DQFGFNDEEF ADQDDNINAP FDRIAEINFN IDADEDSPSA ALFEACCSDR601IQPFDDDEDE DIWEDSDTRC AARVMARPRF GAPHASESCS KNGPERGGQD GKASLEAHRD661APGAGAPPAP GKKEAPPVEG DSEGAMWTAV FDEPANSTPT APGVVRDVGS SVWAAGTSAP721EEKGWAKFTD FQPFCCSESG PRCSSPVDTE CSHAEGSRSQ GPEKAFSPAS PCAWNVCVTR781KAPLLASDSS SSGGSHSEDG DQKAASAMDA VSRGPGREAP PLPTVARTEE AVGRVGCADS841RLLSPACPAP KEVTAAPAVA VPPEATVAIT TALSKAGPAI PTPAVSSALA VAVPLGPIMA901VTAAPAMVAT LGTVTKDGKT DAPPEGAALN GPVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
933 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.