Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PPP6R1 / SAPS1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113603-100
Catalog Number: LS-G113603
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SAPS1(PPP6R1) (NM_014931), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PP6R1 | SAPS domain family | member 1 | SAPS1 | KIAA1115 | PPP6R1 | SAP190 | SAPS domain family member 1
- Origin Species
- Human
- NCBI GenBank
- AB029038
- SwissProt
- Q9UPN7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 97.79 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSRRPPSQGA MFWKFDLHTS SHLDTLLERE DLSLPELLDE EDVLQECKVV NRKLLDFLLQ61PPHLQAMVAW VTQEPPDSGE ERLRYKYPSV ACEILTSDVP QINDALGADE SLLNRLYGFL121QSTGSLNPLL ASFFSKVMGI LINRKTDQLV SFLRKKDDFV DLLLQHIGTS AIMDLLLRLL181TCVERPQLRQ DVVNWLNEEK IVQRLIEQIH PSKDENQHSN ASQSLCDIIR LSREQMIQVQ241DSPEPDQLLA TLEKQETIEQ LLSNMFEGEQ SQSVIVSGIQ VLLTLLEPRR PRSESVTVNS301FFSSVDGQLE LLAQGALEST VSSVGALHAL RPRLSCFHQL LLEPPKLEPL QMTWGMLAPP361LGNTRLHVVK LLASALSAND AALTHELLAL DVPNTMLDLF FHYVFNNFLH AQVEGCVSTM421LSLGPPPDSS PETPIQNPVV KHLLQQCRLV ERILTSWEEN DRVQCAGGPR KGYMGHLTRV481AGALVQNTEK GPNAEQLRQL LKELPSEQQE QWEAFVSGPL AETNKKNMVD LVNTHHLHSS541SDDEDDRLKE FNFPEEAVLQ QAFMDFQMQR MTSAFIDHFG FNDEEFGEQE ESVNAPFDKT601ANITFSLNAD DENPNANLLE ICYKDRIQQF DDDEEEEDEE EAQGSGESDG EDGAWQGSQL661ARGARLGQPP GVRSGGSTDS EDEEEEDEEE EEDEEGIGCA ARGGATPLSY PSPGPQPPGP721SWTATFDPVP TDAPTSPRVS GEEELHTGPP APQGPLSVPQ GLPTQSLASP PARDALQLRS781QDPTPPSAPQ EATEGSKVTE PSAPCQALVS IGDLQATFHG IRSAPSSSDS ATRDPSTSVP841ASGAHQPPQT TEGEKSPEPL GLPQSQSAQA LTPPPIPNGS APEGPASPGS QTRTRPLEQKLISEEDLAANDILDYKDDDDKV
891 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.