Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PPP4R1 / PP4R1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116159-100
Catalog Number: LS-G116159
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PP4R1(PPP4R1) (NM_005134), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MEG1 | PP4R1 | PPP4R1 | PP4(Rmeg) | Serine/threonine phosphatase 4
- Origin Species
- Human
- NCBI GenBank
- NM_005134
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 105 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MADLSLLQED LQEDADGSLD FVSQDEMLTP LGRLDKYAAS ENIFNRQMVA RSLLDTLREV61CDDERDCIAV LERISRLADD SEPTVRAELM EQVPHIALFC QENRPSIPYA FSKFLLPIVV121RYLADQNNQV RKTSQAALLA LLEQELIERF DVETKVCPVL IELTAPDSND DVKTEAVAIM181CKMAPMVGKD ITERLILPRF CEMCCDCRMF HVRKVCAANF GDICSVVGQQ ATEEMLLPRF241FQLCSDNVWG VRKACAECFM AVSCATCQEI RRTKLSALFI NLISDPSRWV RQAAFQSLGP301FISTFANPSS SGQYFKEESK SSEEMSVENK NRTRDQEAPE DVQVRPEDTP SDLSVSNSSV361ILENTMEDHA AEASGKPLGE ISVPLDSSLL CTLSSESHQE AASNENDKKP GNYKSMLRPE421VGTTSQDSAL LDQELYNSFH FWRTPLPEID LDIELEQNSG GKPSPEGPEE ESEGPVPSSP481NITMATRKEL EEMIENLEPH IDDPDVKAQV EVLSAALRAS SLDAHEETIS IEKRSDLQDE541LDINELPNCK INQEDSVPLI SDAVENMDST LHYIHSDSDL SNNSSFSPDE ERRTKVQDVV601PQALLDQYLS MTDPSRAQTV DTEIAKHCAY SLPGVALTLG RQNWHCLRET YETLASDMQW661KVRRTLAFSI HELAVILGDQ LTAADLVPIF NGFLKDLDEV RIGVLKHLHD FLKLLHIDKR721REYLYQLQEF LVTDNSRNWR FRAELAEQLI LLLELYSPRD VYDYLRPIAL NLCADKVSSV781RWISYKLVSE MVKKLHAATP PTFGVDLINE LVENFGRCPK WSGRQAFVFV CQTVIEDDCL841PMDQFAVHLM PHLLTLANDR VPNVRVLLAK TLRQTLLEKD YFLASASCHQ EAVEQTIMAL901QMDRDSDVKY FASIHPASTK ISEDAMSTAS STYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
933 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.