Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PPP3CC / CALNA3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100605-100
Catalog Number: LS-G100605
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPP3CC (NM_005605), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CALNA3 | CNA3 | Calcineurin A gamma | PPP3CC | Phosphatase 2b gamma | PP2Bgamma | CAM-PRP catalytic subunit | CNA-gamma | pp2b gamma
- Origin Species
- Human
- NCBI GenBank
- NM_005605
- SwissProt
- P48454
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSGRRFHLST TDRVIKAVPF PPTQRLTFKE VFENGKPKVD VLKNHLVKEG RLEEEVALKI61INDGAAILRQ EKTMIEVDAP ITVCGDIHGQ FFDLMKLFEV GGSPSNTRYL FLGDYVDRGY121FSIECVLYLW SLKINHPKTL FLLRGNHECR HLTDYFTFKQ ECRIKYSEQV YDACMETFDC181LPLAALLNQQ FLCVHGGMSP EITSLDDIRK LDRFTEPPAF GPVCDLLWSD PSEDYGNEKT241LEHYTHNTVR GCSYFYSYPA VCEFLQNNNL LSIIRAHEAQ DAGYRMYRKS QATGFPSLIT301IFSAPNYLDV YNNKAAVLKY ENNVMNIRQF NCSPHPYWLP NFMDVFTWSL PFVGEKVTEM361LVNVLNICSD DELISDDEAE GSTTVRKEII RNKIRAIGKM ARVFSILRQE SESVLTLKGL421TPTGTLPLGV LSGGKQTIET ATVEAVEARE AIRGFSLQHK IRSFEEARGL DRINERMPPR481KDSIHAGGPM KSVTSAHSHA AHRSDQGKKA HSTRTRPLEQ KLISEEDLAA NDILDYKDDD541DKV
543 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.