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PPP2R5D Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PPP2R5D Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PPP2R5D antibodies →
$725.00each
Catalog Number: LS-G104582-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPP2R5D (NM_180977), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
B56D | Male-enhanced antigen-1 | PP2A B subunit isoform B-delta | PPP2R5D
Origin Species
Human
NCBI GenBank
NM_006245
SwissProt
Q14738

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPYKLKKEKE LFIQKLRQCC VLFDFVSDPL SDLKFKEVKR AGLNEMVEYI THSRDVVTEA
61IYPEAVTMFS VNLFRTLPPS SNPTGAEFDP EEDEPTLEAA WPHLQLVYEF FLRFLESPDF
121QPNIAKKYID QKFVLALLDL FDSEDPRERD FLKTILHRIY GKFLGLRAYI RRQINHIFYR
181FIYETEHHNG IAELLEILGS IINGFALPLK EEHKMFLIRV LLPLHKVKSL SVYHPQLAYC
241VVQFLEKESS LTEPVIVGLL KFWPKTHSPK EVMFLNELEE ILDVIEPSEF SKVMEPLFRQ
301LAKCVSSPHF QVAERALYYW NNEYIMSLIS DNAARVLPIM FPALYRNSKS HWNKTIHGLI
361YNALKLFMEM NQKLFDDCTQ QYKAEKQKGR FRMKEREEMW QKIEELARLN PQYPMFRAPP
421PLPPVYSMET ETPTAEDIQL LKRTVETEAV QMLKDIKKEK VLLRRKSELP QDVYTIKALE
481AHKRAEEFLT ASQEAL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

496 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.